《植物生理学报》 2009, 45(5): 479-482
通信作者:刘胜毅;E-mail: liusy@oilcrops.cn;Tel: 027 -86812896
摘 要:
采用RT-PCR 方法检测茉莉酸(JA)/乙烯(ET)依赖性信号途径中关键基因PDF1.2 在转草酸氧化酶基因(OXO)油菜株系与未转化对照中的表达差异。结果表明, 在转基因油菜不同株系中PDF1.2都有不同程度的上调表达, 预示着转OXO油菜对菌核病的抗性增强可能与激活JA/ET依赖性信号途径有关关键词:甘蓝型油菜; 菌核病; PDF1.2
收稿:2009-03-09 修定:2009-04-28
资助:国家自然科学基金(30671344、30800748)和中国博士后科学基金(20070411080)。
Corresponding author: LIU Sheng-Yi; E-mail: liusy@oilcrops.cn; Tel: 027 -86812896
Abstract:
The expressions of PDF1.2, a key gene in the jasmonic acid (JA)/ethylene (ET)-dependent signaling pathway, were measured in oxalate oxidase (OXO) transgenic and non-transgenic oilseed rapes (Brassica napus) by RT-PCR. The results showed that the expression of PDF1.2 was up-regulated in different OXO transgenic oilseed rape lines, which indicated that the mechanism of disease resistance to Sclerotinia sclerotiorum was related to the induction of the JA/ET-dependent signaling pathway.Key words: oilseed rape (Brassica napus); Sclerotinia sclerotiorum; PDF1.2
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